Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (W303)
Loading amount
2e+006 cells
Specification
W303
Treatment
release into 200mM HU
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% mik in TBST
Secondary antibody
Name
Dilution
1/2000
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 92 kDa
Positive control
WT (G1, 20', 40', 60' +/-HU)
Negative control
sm1 delta rad53 delta (G1, 20', 40', 60' +/-HU)
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投稿 Jul 31 2012