Rabbit IgG secondary antibody - H&L (ab6721)

Product Name 

Rabbit IgG secondary antibody - H&L

Product type 

Secondary antibodies

Description 

Goat polyclonal to Rabbit IgG - H&L (HRP)

Immunogen 

Rabbit IgG, whole molecule

Reacts with 
(species key)

Rb

Tested applications 
(see key)

Dot, ELISA, IHC-Fr, IHC-P, IM, WB

Application notes 
(see key)

Recommended dilutions
Dot: Use at an assay dependent concentration.
ELISA: Use at 1:120,000 dilution
IHC-P: Use at 1:1000 dilution
IHC-Fr: Use at 1:1000 dilution.
IM: Use at an assay dependent concentration.
WB: Suggested working dilution of 1/3000 (see PMID: 17222046). In addition, found to work at 1/20000 (see PMID: 16936283).

Working dilutions are highlighted in the table below. Please note that the antibody can be diluted to 1:48,000 to 1:207,000 in many instances.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Please refer to the relevant conjugation for dilutions specific for this product:

Cellular localization 

Secreted

Research areas 

Secondary antibodies >> anti-Rabbit >> IgG >> Enzyme >> HRP
Immunology >> Immunoglobulins >> Heavy Chain >> IgG

Relevance 

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10¹¹ variants.

Raised in 

Goat

Clonality 

Polyclonal

Purity 

IgG fraction

Conjugation notes 

Horseradish Peroxidase (HRP)

Storage buffer 

Preservative: 0.01% Gentamicin Sulfate
Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2

Purification notes 

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.

Form 

Liquid

Conjugation 

Horse Radish Peroxidase

Concentration 

2.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

References for Rabbit IgG secondary antibody - H&L (ab6721)

  This product has been used in: (two most recent references)

Mehedint MG et al. Choline deficiency alters global histone methylation and epigenetic marking at the Re1 site of the calbindin 1 gene. FASEB J 24:184-95 (2010). WB. PubMed: 19752176

Trang P et al. Regression of murine lung tumors by the let-7 microRNA. Oncogene : (2009). IHC-P. PubMed: 19966857

See all 39 publication references for this product.


If you publish research using ab6721 please let us know so that we can cite the reference on this datasheet.

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