|
|
|
|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG antibody - H&L (ab6720)
(enlarge)
|
|
|
|
|
Product Name
|
Rabbit IgG secondary antibody - H&L
|
|
Product type
|
Secondary antibodies
|
|
Description
|
Goat polyclonal to Rabbit IgG - H&L (Biotin)
|
|
Immunogen
|
Rabbit IgG whole molecule
|
|
Tested applications
(see key)
|
Dot, ELISA, IHC-Fr, IHC-P, IHC-P, IM, WB
|
|
Application notes
(see key)
|
Recommended dilutions
Dot: Use at an assay dependent concentration.
ELISA: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent concentration.
IHC-P: 1/200 see reference Pubmed: 17041957).
IM: Use at an assay dependent concentration.
WB: Use at an assay dependent concentration.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Please refer to the relevant conjugation for dilutions specific for this product:
Conjugate |
ELISA |
Blotting |
Flow Cytometry |
IHC |
Fluorescein |
nd |
nd |
50 - 200 |
200 - 1,000 |
Rhodamine, Texas RedTM |
nd |
nd |
50 - 200 |
200 - 1,000 |
R-Phycoerythrin |
nd |
nd |
100 - 200 |
nd |
Cyanines |
nd |
nd |
400 |
100 - 1,000 |
Biotin |
5,000 - 20,000 |
3,000 - 10,000 |
nd |
100 - 1,000 |
Peroxidase |
4,000 - 10,000 |
2,000 - 5,000 |
nd |
200 - 2,000 |
Alkaline Phosphatase |
1,000 - 2,000 |
500 - 1,000 |
nd |
100 - 1,000 |
|
|
|
Cellular localization
|
Secreted
|
|
Research areas
|
Secondary antibodies >> anti-Rabbit >> IgG >> Biotin
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
|
|
Relevance
|
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
|
|
|
Rabbit IgG secondary antibody - H&L (ab6720)の別名 |
|
Raised in
|
Goat
|
|
Clonality
|
Polyclonal
|
|
Purity
|
IgG fraction
|
|
Conjugation notes
|
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
|
|
Storage buffer
|
0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Purification notes
|
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
|
|
Form
|
Liquid
|
|
Conjugation
|
Biotin
|
|
Concentration
|
2.000 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
Abcam では全ての製品情報を一元管理しており、 Rabbit IgG secondary antibody - H&L について私たちが知りうる全ての情報がこのデータシートでご覧いただけます。ご不明な点がありましたら、 お問い合わせください。 |
 |
|
| |
Rabbit IgG secondary antibody - H&L images:
|
 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG antibody - H&L (ab6720)
ab28829 (Rabbit polyclonal to STAT6 (phospho Y641)) at 1/100 staining mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and blocked with serum. A heat mediated antigen retrieval step was performed prior to incubation with the antibody. Ab6720 (Goat polyclonal to Rabbit IgG H&L (biotin)) was used as the secondary antibody. This image is courtesy of an anonymous Abreview
See Abreview
|
| |
References for Rabbit IgG secondary antibody - H&L (ab6720)
|
|
This product has been used in: (two most recent references)
McEgan R et al. Concentration and detection of Salmonella in mung bean sprout spent irrigation water by use of tangential flow filtration coupled with an amperometric flowthrough enzyme-linked immunosorbent assay. J Food Prot 72:591-600 (2009). PubMed: 19343949
Novella-Maestre E et al. Dopamine agonist administration causes a reduction in endometrial implants through modulation of angiogenesis in experimentally induced endometriosis. Hum Reprod 24:1025-35 (2009). PubMed: 19189995
See all 5 publication references for this product.
If you publish research using ab6720 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to IgG
|
| |
Rabbit IgG secondary antibody - H&L - more information
|
Customer reviews (feedback) regarding Rabbit IgG secondary antibody - H&L |
Customer FAQs regarding Rabbit IgG secondary antibody - H&L |
Protocols for Rabbit IgG secondary antibody - H&L |
Price and availability of products related to Rabbit IgG secondary antibody - H&L |
| |
Rabbit IgG secondary antibody - H&L - other tools:
|
Contact Abcam with a Technical Enquiry about Rabbit IgG secondary antibody - H&L |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
