Rabbit IgG secondary antibody - H&L (ab6564)

Product Name 

Rabbit IgG secondary antibody - H&L

Product type 

Secondary antibodies

Description 

Goat polyclonal to Rabbit IgG - H&L (Cy5 ®)

Immunogen 

Rabbit IgG, whole molecule

Species Reactivity 

No reaction is observed against Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rat and Sheep Serum Proteins.

Tested applications 
(see key)

Flow Cyt, ICC/IF, IHC-Fr, IHC-P

Application notes 
(see key)

Recommended dilutions
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Please refer to the relevant conjugation for dilutions specific for this product:

Cellular localization 

Secreted

Research areas 

Secondary antibodies >> anti-Rabbit >> IgG >> Fluorophore >> Cy5
Immunology >> Immunoglobulins >> Heavy Chain >> IgG

Relevance 

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10¹¹ variants.

This secondaryantibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

Raised in 

Goat

Clonality 

Polyclonal

Purity 

Immunogen affinity purified

Conjugation notes 

Cy5.29 (Cyanine 5.29-OSu) (Molecular Weight 975 daltons)
Absorption Wavelength: 650 nm
Emission Wavelength: 667 nm
Fluorochrome/Protein Ratio: 6.0 moles Cy5 per mole of Goat IgG

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis.

Form 

Liquid

Conjugation 

Cy5 ®

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for Rabbit IgG secondary antibody - H&L (ab6564)

  This product has been used in: (two most recent references)

Delobel P et al. Cell-cycle markers in a transgenic mouse model of human tauopathy: increased levels of cyclin-dependent kinase inhibitors p21Cip1 and p27Kip1. Am J Pathol 168:878-87 (2006). PubMed: 16507903

Schulte J et al. Gliotactin and Discs large form a protein complex at the tricellular junction of polarized epithelial cells in Drosophila. J Cell Sci 119:4391-401 (2006). PubMed: 17032735
If you publish research using ab6564 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding Rabbit IgG secondary antibody - H&L

Customer FAQs regarding Rabbit IgG secondary antibody - H&L

Protocols for Rabbit IgG secondary antibody - H&L

Price and availability of products related to Rabbit IgG secondary antibody - H&L