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Products:Immunology >> Immunoglobulins >> Heavy Chain >> IgG
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy5 ®), pre-adsorbed (ab6564)
Overview
Product name
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy5 ®), pre-adsorbed
IgG 抗体 (1691件) 一覧
Target species
Rabbit
Tested applications
Flow Cyt, ICC/IF, ELISA, IHC-P, IHC-Frmore details
Minimal
cross-reactivity
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheepmore details
Immunogen
Rabbit IgG, whole molecule
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis.
Conjugation notes
Cy5.29 (Cyanine 5.29-OSu) (Molecular Weight 975 daltons) Absorption Wavelength: 650 nm Emission Wavelength: 667 nm Fluorochrome/Protein Ratio: 6.0 moles Cy5 per mole of Goat IgG
Clonality
Polyclonal
General notes
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
This material is also subject to proprietary rights of GE Healthcare
Bio-Sciences Corp. and Carnegie Mellon University and made and
sold under License from GE Healthcare Bio-Sciences Corp. This
product is licensed for sale only for research. It is NOT licensed for
any other use. There is no implied license hereunder for any
commercial use.
COMMERCIAL USE shall include:
1 Sale, lease, license or other transfer of the material or any material
derived or produced from it.
2 Sale, lease, license or other grant of rights to use this material or any
material derived or produced from it.
3 Use of this material to perform services for a fee for third parties.
If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.
Research areas
Secondary antibodies >> anti-Rabbit >> IgG >> Fluorophore >> Cy5
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab6564 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: 1/500 - 1/2500.
ICC/IF: 1/1000 - 1/5000.
ELISA: 1/10000 - 1/50000.
IHC-P: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent concentration.
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy5 ®), pre-adsorbed images:
Flow Cytometry - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy5 ®), pre-adsorbed (ab6564)
Ab6564 was used at dilution 1/300 with the primary antibody ab19898 in Flow Cyt. See the review on ab19898.
Immunocytochemistry/ Immunofluorescence - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy5 ®), pre-adsorbed (ab6564)
Ab6564 was used at dilution 1/500 with the primary antibody ab24586 in ICC/IF. See the review on ab24586.
References for Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy5 ®), pre-adsorbed (ab6564)
This product has been referenced in:
- Fatakdawala H & Uhland SA Hydrogen peroxide mediated transvaginal drug delivery. Int J Pharm 409:121-7 (2011). ICC/IF; Human.Read more (PubMed: 21498011) »
- Tavernier Get al. Activation of pluripotency-associated genes in mouse embryonic fibroblasts by non-viral transfection with in vitro-derived mRNAs encoding Oct4, Sox2, Klf4 and cMyc. Biomaterials : (2011). ICC/IF.Read more (PubMed: 21993235) »
See all 6 publications for this product
Publishing research using ab6564? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"