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Product Name 

Rabbit Control IgG - ChIP Grade

Product type 

Isotype controls

Description 

Rabbit Control IgG - ChIP Grade

Immunogen 

Native, mouse whole IgG.

Specificity 

Assay by IEP resulted in a single precipitin arc against anti-rabbit serum, mouse IgG and mouse serum.

Tested applications 
(see key)

ChIP, ELISA, WB

Application notes 
(see key)

Recommended dilutions
ChIP: Use at an assay dependent dilution.
ELISA: 1/20000 - 1/100000.
WB: 1/2000 - 1/10000.

This product is Suitable for IPPT, immunodiffusion and most immunological methods requiring lot-to-lot consistency, high titre and specificity.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.



Specific protocols
X-ChIP

Cellular localization 

Secreted

Research areas 

Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Immunology >> Immunoglobulins >> Heavy Chain >> IgG

Relevance 

IgG is the most abundant class of antibodies found in blood serum and lymph. It is active against bacteria, fungi, viruses, and foreign particles. Immunoglobulin G antibodies trigger action of the complement system.

 

Rabbit Control IgG - ChIP Grade (ab46540)の別名

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

This product was purified by affinity chromatography using mouse IgG-agarose.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

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References for Rabbit Control IgG - ChIP Grade (ab46540)

Specific publication references   This product has been used in: (two most recent references)

Hughes S et al. Transcriptional co-activator LEDGF interacts with Cdc7-activator of S-phase kinase (ASK) and stimulates its enzymatic activity. J Biol Chem 285:541-54 (2010). IP. PubMed: 19864417

Verdeguer F et al. A mitotic transcriptional switch in polycystic kidney disease. Nat Med 16:106-10 (2010). ChIP. PubMed: 19966811


See all 11 publication references for this product.


If you publish research using ab46540 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to Mouse IgG

 

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