Application
Western blot
Sample
Human Cell lysate - other (HeLa)
Loading amount
100000 cells
Specification
HeLa
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Other product details
Dilution
1/2000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C
Secondary antibody
Name
Non-Abcam antibody was used: swine anti rabbit HRP
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL
Exposure
1 hour(s), 30 minute(s) and 0 second(s)
Bands
Specific: 24 kDa
Negative control
Nuclear fraction
Additional data
Additional Notes
Lane 1 = cytosolic fraction from HeLa cells
Lane 2 = nuclear fraction from HeLa cells (negative control)
Lane 2 = nuclear fraction from HeLa cells (negative control)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Mar 31 2009