Anti-Rab5 抗体 [EPR5438] - Early Endosome Marker (ab109534)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5438] to Rab5 - Early Endosome Marker
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Rab5 antibody [EPR5438] - Early Endosome Marker
Rab5 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5438] to Rab5 - Early Endosome Marker -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- 293T, HeLa and Jurkat whole cell lysate (ab7899); Human brain tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5438 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109534の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (5) |
1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
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IHC-P | (1) |
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 24 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
ターゲット情報
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機能
Required for the fusion of plasma membranes and early endosomes. -
配列類似性
Belongs to the small GTPase superfamily. Rab family. -
細胞内局在
Cell membrane. Early endosome membrane. Melanosome. Enriched in stage I melanosomes. - Information by UniProt
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参照データベース
- Entrez Gene: 5868 Human
- Entrez Gene: 271457 Mouse
- Entrez Gene: 64633 Rat
- Omim: 179512 Human
- SwissProt: P20339 Human
- SwissProt: Q9CQD1 Mouse
- SwissProt: M0RC99 Rat
- Unigene: 475663 Human
see all -
別名
- RAB 5 antibody
- RAB 5A antibody
- RAB5A antibody
see all
画像
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Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Rab5 knockout HAP1 whole cell lysate (20 µg)
Lane 3: Hu brain whole cell lysate (20 µg)
Lane 4: Ms brain whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109534 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa. -
Immunohistochemical staining of paraffin embedded human pancreas with purified ab109534 at a working dilution of 1 in 1000. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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All lanes : Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534) at 1/5000 dilution (purified)
Lane 1 : Mouse brain
Lane 2 : Rat brain
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Pancreas tissue sections (with ADM and PanIN lesions) from KC mice labeling Rab5 with ab109534 at 1/200 dilution. Tissue was fixed with paraformaldehyde and permeabilized with TritonX100 0.1% for 10 min at room temperature. Heat-mediated antigen retrieval was performed using TRIS-EDTA pH9 buffer. Tissue sections were blocked with 10% goat serum for 1 hour at 20°C. A polyclonal goat anti-rabbit Alexa Fluor 488 secondary antibody was used at 1/500 dilution.
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All lanes : Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534) at 1/5000 dilution (purified)
Lane 1 : MCF7 cell lysate
Lane 2 : HEK293 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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All lanes : Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534) at 1/1000 dilution (unpurified)
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 24 kDa -
Immunohistochemical staining of Rab5 in paraffin embedded human brain tissue with unpurified ab109534, at 1/100 dilution.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab109534 は 15 報の論文で使用されています。
- Zhou G et al. Exosome Mediated Cytosolic Cisplatin Delivery Through Clathrin-Independent Endocytosis and Enhanced Anti-cancer Effect via Avoiding Endosome Trapping in Cisplatin-Resistant Ovarian Cancer. Front Med (Lausanne) 9:810761 (2022). PubMed: 35592860
- Andersen OM et al. A genetically modified minipig model for Alzheimer's disease with SORL1 haploinsufficiency. Cell Rep Med 3:100740 (2022). PubMed: 36099918
- Frittoli E et al. Tissue fluidification promotes a cGAS-STING cytosolic DNA response in invasive breast cancer. Nat Mater N/A:N/A (2022). PubMed: 36581770
- Sharoar MG et al. Accumulation of saposin in dystrophic neurites is linked to impaired lysosomal functions in Alzheimer's disease brains. Mol Neurodegener 16:45 (2021). PubMed: 34215298
- Stoffel W et al. High fat / high cholesterol diet does not provoke atherosclerosis in the ω3-and ω6-polyunsaturated fatty acid synthesis-inactivated Δ6-fatty acid desaturase-deficient mouse. Mol Metab 54:101335 (2021). PubMed: 34530175