Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Fruit fly (Drosophila melanogaster) Cell (larval fat body)
Specification
larval fat body
Permeabilization
Yes - PBS + 0.1% Triton X-100
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 0.1% Triton-X100 + 5%serum
Dilution
1/300
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3
Dilution
1/1000
Additional data
Additional Notes
Clonal silencing of Rab5 were performed (FLPout system, the green cells correspond to Rab5 RNAi expressing cells, neighbor are wild-type cells) and the fat body of third instar larvae were dissected.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Anne-Claire Jacomin
Verified customer
投稿 Aug 30 2013