Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Rat brain)
Specification
Rat brain
Fixative
Paraformaldehyde
Permeabilization
No
Other product details
Dilution
1/300
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBS triton 0.3%
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/2000
Additional data
Additional Notes
Adult rat was perfused intracardially with paraformaldehyde 4% in PB 0.2M. Sections were cryoprotected with sucrose 20% and later frozen in OCT. Sections were incubated in free floating 12h with the primary antibody (1:300 diluted) and revealed with secondary antibody conjugated with Alexa Fluor 488 (1:2000).
The staining obtained is restricted to the neuronal cytoplasm. The picture shows the staining obtained at the level of cortical neurons.
The staining obtained is restricted to the neuronal cytoplasm. The picture shows the staining obtained at the level of cortical neurons.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Sophie Pezet
Verified customer
投稿 Aug 28 2007