Application
Western blot
Sample
Mouse Tissue lysate - whole (thoracic-lumbar spinal cord)
Loading amount
1 µg
Specification
thoracic-lumbar spinal cord
Gel Running Conditions
Reduced Denaturing (Invitrogen 4-12% NP0323)
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 2% · Temperature: RT°C
Other product details
Dilution
1/500
Incubation time
17 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST BSA2%
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit HRP (Cell signaling cat number 7074)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL BioRAD cat170-5070
Exposure
1 minute(s) and 0 second(s)
Positive control
I used other lanes in the same gel to test another antibody anti-Rab11 and as expected it produced a band at ~25Kdal.
B-tubulin was good and similar in all lanes of this gel.
B-tubulin was good and similar in all lanes of this gel.
Additional data
Abcam response
We are thankful for customer's collaboration in testing this antibody in Mouse.
Black blot/dots indicate a reaction between the antibodies and blocking agent. We have not had similar complaints.
Different blocking agents, secondary antibody could be tried to see if the problem is with the primary antibody vial.
We welcome any feedback regarding mouse suitability from customers!
Black blot/dots indicate a reaction between the antibodies and blocking agent. We have not had similar complaints.
Different blocking agents, secondary antibody could be tried to see if the problem is with the primary antibody vial.
We welcome any feedback regarding mouse suitability from customers!
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Thomas Sardella
Verified customer
投稿 Feb 01 2011