Application
Western blot
Sample
Human Cell lysate - whole cell (A431 Cells)
Loading amount
5 µg
Specification
A431 Cells
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Concentration
1 µg/ml
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 2.5% Milk + 0.05% Tween20
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 24 kDa Non-specific: 75, 48, 52 kDa
Additional data
Additional Notes
The identity of the additional bands observed is unknown
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jun 15 2010