Application
Western blot
Loading amount
10000 cells
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Human Cell lysate - whole cell (293T)
Specification
293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Concentration
0.5 µg/ml
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in PBS/Tween
Secondary antibody
Name
Non-Abcam antibody was used: Jackson laboratories anti-mouse heavy and light ch
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Pierce Pico
Bands
Specific: 45, 48 kDa
Exposure
5 second(s)
Positive control
Lane 1. 293T cells
Lane 2. 293T transiently transfected with RRM2 expression vector
Additional data
Additional Notes
My findings differ from that shown in the datasheet - expected band is 45kDa, while datasheet shows suggests specific band is below 37kDa, with a non-specific band between 37kDa and 50kDa. I see no bands below 37kDa, while specific bands are between 37kDa and 50kDa as expected.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 20 2014