Application
Western blot
Loading amount
10000 cells
Gel Running Conditions
Reduced Denaturing (8%)
Sample
African Green Monkey Cell lysate - whole cell (COS-7 - AGM kidney tissue fibroblast like line)
Specification
COS-7 - AGM kidney tissue fibroblast like line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Concentration
0.5 µg/ml
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in PBS/Tween
Secondary antibody
Name
Non-Abcam antibody was used: Jackson laboratories anti-mouse heavy and light ch
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000
Detection
Detection method
Pierce Pico
Bands
Specific: 45, 48 kDa Non-specific: only when over-expos kDa
Exposure
5 second(s)
Additional data
Additional Notes
ABTRIAL-EBLC5
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 19 2014