Application
Western blot
Sample
Caenorhabditis elegans Tissue lysate - whole (whole adult)
Gel Running Conditions
Reduced Denaturing
Loading amount
200 µg
Specification
whole adult
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Can Get Signal Solution 1
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Goat HRP anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL Prime
Negative control
none
Exposure
40 second(s)
Bands
Specific: 28 kDa Non-specific: 24, 26, 40 kDa
Positive control
none
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Mar 27 2014