Application
Immunocytochemistry/ Immunofluorescence
Sample
Drosophila melanogaster Cell (S2 D.mel embryonic cell line)
Permeabilization
Yes - 0.05% Triton X100
Specification
S2 D.mel embryonic cell line
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 0.05% Triton X100
Dilution
1/100
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Secondary Antibody, Ale
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Additional data
Additional Notes
Nice dot-like signal but tends to aggregate at moderate to high concentrations.
Drosophila S2 cells were fixed with 4% PFA in PBS for 15min and then permeabilized with 3 washes in PBT (0.05% Triton X100 in PBS). Primary antibody was directly incubated with cells at 1/100 concentration in PBT overnight at 4C. Cells were washed again with PBT and conjugated secondary antibody was incubated in PBT for 4hrs at RT.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Kelly Burke
Verified customer
投稿 Jul 22 2015