Application
Western blot
Sample
Zebrafish Tissue lysate - whole (whole larvae)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
4% glucose, 4% fructose, 4% fructose + 100 nM Rapamycin
Specification
whole larvae
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-T (1x TBS +.1% Tween-20)
Dilution
1/200
Secondary antibody
Dilution
1/50000
Name
Non-Abcam antibody was used: donkey anti rabbit HRP conjugated
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: none kDa Non-specific: 20, 15, <10 kDa
Additional data
Additional Notes
I also used this antibody for IHC--IF on patient liver samples and it worked well for that application.
Abcam response
Thank you for submitting this Abreview. As this antibody hasn't been validated for zebrafish samples, we are unsure if the antibody is suitable for this species. It may require further optimization.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Nov 13 2013