Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS cells)
Gel Running Conditions
Reduced Denaturing (13% gel)
Loading amount
10 µg
Treatment
RPL7 siRNA
Specification
U2OS cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 18°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% milk
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
RPL7 siRNAs used
Exposure
10 second(s)
Bands
Specific: ~30 kDa
Positive control
Control siRNA used
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mrs. Loren Gibson
Verified customer
投稿 May 20 2015