Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (gradient)
Sample
Human Cell lysate - whole cell (U2OS cells)
Specification
U2OS cells
Treatment
Time course of serum stimulation
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: DAKO
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
West Femto
Bands
Specific: 15 kDa Non-specific: 130 kDa
Exposure
10 minute(s) and 0 second(s)
Additional data
Additional Notes
1. Control
2. 4h serum
3. 8h serum
4. 16h serum
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jun 13 2013