Application
Western blot
Sample
Mouse Tissue lysate - whole (Forebrain)
Loading amount
10 µg
Specification
Forebrain
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Odyssey blocking buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: IRDye 800CW Goat anti-Rabbit IgG (H + L)
Host species: Rabbit
Clonality: Monoclonal
Conjugation: IRDye® 800CW
Host species: Rabbit
Clonality: Monoclonal
Conjugation: IRDye® 800CW
Dilution
1/20000
Detection
Detection method
Licor
Exposure
1 minute(s) and 0 second(s)
Positive control
Sample was heterozygous for tagged RPL22. Tag was detected but endogenous protein was not. Tuj1 was detected in the samples.
Additional data
Abcam response
We would like to thank the reviewer for submitting this Abreview for this product. As the product had not yet been tested in this application on this tissue type we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application. We encourage all our customers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Feb 14 2013