Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Permeabilization
Yes - Triton 0.3%
Specification
U2OS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking
Dilution
1/500
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Alexa 488
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Additional data
Additional Notes
pRPA foci in the site of DSBs due to rereplication.
before PFA 4% fixation, pre-extraction is needed
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jan 24 2013