Anti-RPA32/RPA2 抗体 (ab10359)
Key features and details
- Rabbit polyclonal to RPA32/RPA2
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-RPA32/RPA2 antibody
RPA32/RPA2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RPA32/RPA2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rabbit, Horse, Pig, Chimpanzee, Rhesus monkey, Gorilla, Bat, Elephant -
免疫原
Synthetic peptide corresponding to Human RPA32/RPA2 aa 200 to the C-terminus (C terminal).
Database link: P15927 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10359の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/500 - 1/2500. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
|
|
IP |
Use at an assay dependent concentration.
|
|
IHC-P |
Use at an assay dependent concentration.
|
特記事項 |
---|
WB
1/500 - 1/2500. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). |
IP
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
-
機能
Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. -
翻訳後修飾
Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1. -
細胞内局在
Nucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage. - Information by UniProt
-
参照データベース
- Entrez Gene: 456680 Chimpanzee
- Entrez Gene: 101144875 Gorilla
- Entrez Gene: 6118 Human
- Entrez Gene: 19891 Mouse
- Entrez Gene: 100347550 Rabbit
- Entrez Gene: 716612 Rhesus monkey
- Omim: 179836 Human
- SwissProt: P15927 Human
see all -
別名
- 60S acidic ribosomal protein P1 antibody
- AA409079 antibody
- AI325195 antibody
see all
画像
-
All lanes : Anti-RPA32/RPA2 antibody (ab10359) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : Mouse TCMK-1 whole cell lysate
Lane 5 : Mouse NIH 3T3 whole cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 32 kDa
Exposure time: 10 seconds -
HeLa whole cell lysate cells treated with 100 µM etoposide for 4 hours (+) or mock treated (-).
Affinity purified rabbit anti-RPA32 antibody (3 µg) and rabbit anti-Phospho RPA32 antibody (1/100 dilution) were used.
Detection: Chemiluminescence
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling RPA32/RPA2 with ab10359 at 1/5000 (0.2µg/ml). Detection: DAB.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (13)
ab10359 は 13 報の論文で使用されています。
- Fowler FC et al. DNA-PK promotes DNA end resection at DNA double strand breaks in G0 cells. Elife 11:N/A (2022). PubMed: 35575473
- Yang F et al. LACTB induced apoptosis of oxaliplatin-resistant gastric cancer through regulating autophagy-mediated mitochondrial apoptosis pathway. Am J Transl Res 13:601-616 (2021). PubMed: 33594312
- Paiano J et al. Role of 53BP1 in end protection and DNA synthesis at DNA breaks. Genes Dev 35:1356-1367 (2021). PubMed: 34503990
- Shorrocks AK et al. The Bloom syndrome complex senses RPA-coated single-stranded DNA to restart stalled replication forks. Nat Commun 12:585 (2021). PubMed: 33500419
- Callen E et al. 53BP1 Enforces Distinct Pre- and Post-resection Blocks on Homologous Recombination. Mol Cell 77:26-38.e7 (2020). PubMed: 31653568