Application
Western blot
Sample
Human Cell lysate - whole cell (breast cancer cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Treatment
HU 1mM 16hr
Specification
breast cancer cell line
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Ms HRP
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
20 second(s)
Bands
Specific: 30, 45 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 11 2016