Anti-RON (phospho Y1353) 抗体 [EPR5598] (ab124671)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5598] to RON (phospho Y1353)
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-RON (phospho Y1353) antibody [EPR5598]
RON 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5598] to RON (phospho Y1353) -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details
適用なし: Flow Cyt or IHC-P -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- A431 cell lysates treated with pervanadate; A431 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 9.05 x 10 -11 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR5598 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124671の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/50000 - 1/200000. Detects a band of approximately 180 kDa (predicted molecular weight: 152 kDa).
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ICC/IF |
1/500 - 1/1000.
|
特記事項 |
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WB
1/50000 - 1/200000. Detects a band of approximately 180 kDa (predicted molecular weight: 152 kDa). |
ICC/IF
1/500 - 1/1000. |
ターゲット情報
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機能
Receptor for macrophage stimulating protein (MSP). Has a tyrosine-protein kinase activity. -
組織特異性
Keratinocytes and lung. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family.
Contains 3 IPT/TIG domains.
Contains 1 protein kinase domain.
Contains 1 Sema domain. -
翻訳後修飾
Proteolytic processing yields the two subunits.
Phosphorylated in response to ligand binding. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4486 Human
- Omim: 600168 Human
- SwissProt: Q04912 Human
- Unigene: 517973 Human
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別名
- c met related tyrosine kinase antibody
- CD136 antibody
- CD136 antigen antibody
see all
画像
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All lanes : Anti-RON (phospho Y1353) antibody [EPR5598] (ab124671) at 1/50000 dilution
Lane 1 : A431 cell lysates, untreated
Lane 2 : A431 cell lysates, treated with pervanadate
Predicted band size: 152 kDa -
ab124671, at 1/500 dilution, staining RON in A431 (Human epidermoid carcinoma cell line) cells by Immunofluorescence.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab124671 は 2 報の論文で使用されています。
- Song S et al. EGFR/MET promotes hepatocellular carcinoma metastasis by stabilizing tumor cells and resisting to RTKs inhibitors in circulating tumor microemboli. Cell Death Dis 13:351 (2022). PubMed: 35428350
- de la Fuente C et al. Phosphoproteomic analysis reveals Smad protein family activation following Rift Valley fever virus infection. PLoS One 13:e0191983 (2018). PubMed: 29408900