Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Mouse Cell lysate - whole cell (Pei1)
Specification
Pei1
Treatment
Knockdown of Rnaseh2c using shRNA along with scramble control
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Concentration
1 µg/ml
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 5% milk in TBST
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
diluted ECL
Bands
Specific: 18 kDa
Exposure
1 second(s)
Additional data
Additional Notes
Image description:
Lane 1: scramble shRNA
Lane 2: shRNA #1
Lane 3: shRNA #2
Lane 4: shRNA #3
Lane 5: shRNA #4
Left markers: 20 kDa and 15 kDa
Right markers: 25 kDa and 15 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MISS. Sarah Deasy
Verified customer
投稿 May 30 2014