Application
Western blot
Sample
Human Cell lysate - whole cell (A549 Cells)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
100 µg
Specification
A549 Cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: goat anti-rabbit IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
siRNA specific to human HOIP
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 110 kDa Non-specific: 100 kDa
Positive control
Scramble siRNA
Additional data
Additional Notes
lane 1-2 : si CT A549
lane 3-4 : siHOIP A549
upper : IB HOIP
lower: IB Actin
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 10 2018