Anti-RNF144A 抗体 (ab75054)
Key features and details
- Rabbit polyclonal to RNF144A
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RNF144A antibody
RNF144A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RNF144A -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide corresponding to Human RNF144A (internal sequence).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75054の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 33 kDa).
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 33 kDa). |
ターゲット情報
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機能
E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the RBR family. RNF144 subfamily.
Contains 1 IBR-type zinc finger.
Contains 2 RING-type zinc fingers. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 9781 Human
- Entrez Gene: 108089 Mouse
- SwissProt: P50876 Human
- SwissProt: Q925F3 Mouse
- Unigene: 22146 Human
- Unigene: 214932 Mouse
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別名
- KIAA0161 antibody
- Probable E3 ubiquitin protein ligase RNF144A antibody
- Probable E3 ubiquitin-protein ligase RNF144A antibody
see all
画像
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All lanes : Anti-RNF144A antibody (ab75054) at 1/500 dilution
Lane 1 : extracts from COLO cells
Lane 2 : extracts from COLO cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 33 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Additional bands at: 86 kDa. We are unsure as to the identity of these extra bands.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab75054 は 5 報の論文で使用されています。
- Chen X et al. DHHC protein family targets different subsets of glioma stem cells in specific niches. J Exp Clin Cancer Res 38:25 (2019). PubMed: 30658672
- Ho SR & Lin WC RNF144A sustains EGFR signaling to promote EGF-dependent cell proliferation. J Biol Chem 293:16307-16323 (2018). PubMed: 30171075
- Ho SR et al. Regulation of RNF144A E3 Ubiquitin Ligase Activity by Self-association through Its Transmembrane Domain. J Biol Chem 290:23026-38 (2015). PubMed: 26216882
- Ho SR et al. RNF144A, an E3 ubiquitin ligase for DNA-PKcs, promotes apoptosis during DNA damage. Proc Natl Acad Sci U S A 111:E2646-55 (2014). PubMed: 24979766
- Marzook H et al. Metastasis-associated protein 1 drives tumor cell migration and invasion through transcriptional repression of RING finger protein 144A. J Biol Chem 287:5615-26 (2012). WB ; Human . PubMed: 22184113