Application
Western blot
Sample
Mouse Tissue lysate - whole (Multiple Tissues)
Gel Running Conditions
Reduced Denaturing (Bis-Tris 4-12%)
Loading amount
30 µg
Specification
Multiple Tissues
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk
Dilution
1/2000
Secondary antibody
Dilution
1/20000
Name
Non-Abcam antibody was used: IgG HRP Goat anti Rabbit (sc-2054)
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 20 kDa Non-specific: 17 kDa
Positive control
GFP tagged human RNF138 transfected into HeK cells observed at 55kDa
Additional data
Additional Notes
Lanes
1)Spleen:
2)Skin
3)Adipose
4)Testes
5)HeK lysate
6)Lysate of HeK cells transfected with human RNF138 over-expression
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 28 2014