Anti-RNF125 抗体 (ab74373)
Key features and details
- Rabbit polyclonal to RNF125
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RNF125 antibody
RNF125 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RNF125 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab74373の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). |
ターゲット情報
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機能
E3 ubiquitin-protein ligase that acts as a positive regulator of T-cell activation. E3 ligase proteins mediate ubiquitination and subsequent proteasomal degradation of target proteins. -
組織特異性
Predominantly expressed in lymphoid tissues, including bone marrow, spleen and thymus. Also weakly expressed in other tissues. Predominant in the CD4+ and CD8+ T-cells, suggesting that it is preferentially confined to T-cells. -
パスウェイ
Protein modification; protein ubiquitination. -
関連疾患
Tenorio syndrome -
配列類似性
Contains 1 C2HC RNF-type zinc finger.
Contains 1 RING-type zinc finger. -
翻訳後修飾
Autoubiquitinated, leading to its subsequent proteasomal degradation. -
細胞内局在
Golgi apparatus membrane. Shows a reticular staining pattern within the cell and is probably expressed at other intracellular membranes in addition to the Golgi membrane. Not detected at the plasma membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 54941 Human
- Entrez Gene: 361296 Rat
- Omim: 610432 Human
- SwissProt: Q96EQ8 Human
- Unigene: 633703 Human
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別名
- E3 ubiquitin protein ligase RNF125 antibody
- E3 ubiquitin-protein ligase RNF125 antibody
- RING finger protein 125 antibody
see all
画像
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All lanes : Anti-RNF125 antibody (ab74373) at 1/200 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : Mouse ES (129Sv) whole cell lysate
Lysates/proteins at 5 µg per lane.
Secondary
All lanes : HRP-conjugated Donkey anti-rabbit IgG polyclonal at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Additional bands at: 100 kDa (possible non-specific binding)
Exposure time: 1 minute
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All lanes : Anti-RNF125 antibody (ab74373) at 1/500 dilution
Lane 1 : Extracts from K562 cells
Lane 2 : Extracts from HT-29 cells
Lane 3 : Extracts from K562 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Additional bands at: 100 kDa, 23 kDa. We are unsure as to the identity of these extra bands.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab74373 は 2 報の論文で使用されています。
- Ali H et al. Cellular TRIM33 restrains HIV-1 infection by targeting viral integrase for proteasomal degradation. Nat Commun 10:926 (2019). PubMed: 30804369
- Luo H et al. Human bocavirus VP2 upregulates IFN-ß pathway by inhibiting ring finger protein 125-mediated ubiquitination of retinoic acid-inducible gene-I. J Immunol 191:660-9 (2013). PubMed: 23772026