Anti-RNA polymerase II RPB1 抗体 [EPR1509Y] - ChIP Grade (ab76123)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1509Y] to RNA polymerase II RPB1 - ChIP Grade
- Suitable for: WB, ChIP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-RNA polymerase II RPB1 antibody [EPR1509Y] - ChIP Grade
RNA polymerase II RPB1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR1509Y] to RNA polymerase II RPB1 - ChIP Grade -
由来種
Rabbit -
アプリケーション
適用あり: WB, ChIPmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human RNA polymerase II RPB1 aa 100-200 (N terminal). The exact sequence is proprietary.
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ポジティブ・コントロール
- HeLa whole cell lysate (ab150035).
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR1509Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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ChIP Related Products
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76123の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Detects a band of approximately 270 kDa (predicted molecular weight: 217 kDa).
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ChIP |
Use at an assay dependent concentration.
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 270 kDa (predicted molecular weight: 217 kDa). |
ChIP
Use at an assay dependent concentration. |
ターゲット情報
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関連性
RPB1 is a subunit of RNA polymerase II which catalyses transcription of RNA from DNA. RPB1 forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 5430 Human
- Omim: 180660 Human
- SwissProt: P24928 Human
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別名
- DNA directed RNA polymerase II subunit RPB1 antibody
- hRPB220 antibody
- hsRPB1 antibody
see all
画像
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Anti-RNA polymerase II RPB1 antibody [EPR1509Y] - ChIP Grade (ab76123) at 1/2000 dilution + HeLa cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 217 kDa
Observed band size: 270 kDa why is the actual band size different from the predicted? -
Chromatin was prepared from 293T cells according to the Abcam Dual-X-ChIP protocol*. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
The ChIP was performed with 25 µg of chromatin, 5 µg of ab76123 (red), or 5 µg of rabbit normal IgG ab172730 (gray) and 20 µl of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci).
Primers and probes are commercial primers from Paper
*http://www.abcam.com/resources?keywords=X%20ChIP%20protocol
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab76123 は 14 報の論文で使用されています。
- Fan Y et al. PRKDC promotes hepatitis B virus transcription through enhancing the binding of RNA Pol II to cccDNA. Cell Death Dis 13:404 (2022). PubMed: 35468873
- Fasano C et al. Identifying novel SMYD3 interactors on the trail of cancer hallmarks. Comput Struct Biotechnol J 20:1860-1875 (2022). PubMed: 35495117
- Tian Y et al. Transcriptome-wide stability analysis uncovers LARP4-mediated NF?B1 mRNA stabilization during T cell activation. Nucleic Acids Res 48:8724-8739 (2020). PubMed: 32735645
- NandyMazumdar M et al. Looping of upstream cis-regulatory elements is required for CFTR expression in human airway epithelial cells. Nucleic Acids Res 48:3513-3524 (2020). PubMed: 32095812
- Cao L et al. Genotoxic stress-triggered ß-catenin/JDP2/PRMT5 complex facilitates reestablishing glutathione homeostasis. Nat Commun 10:3761 (2019). PubMed: 31434880