Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela cell)
Specification
Hela cell
Fixative
Methanol
Permeabilization
No
Blocking step
Fish scale gelatin as blocking agent for 20 minute(s) · Concentration: 0.2% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
20 minute(s) · Temperature: 25°C · Diluent: 0.2% Fish scale gelatin in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-mouse IgG, Invitrogen (A-21203)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/500
Additional data
Additional Notes
PolII CTD repeat YSPTSPS (phospho S5) is clearly visualized (red channel) in the nuclear of Hela cells by immunostaining using RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody [8A7] (ab5401)
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投稿 Jun 07 2010