Application
Western blot
Sample
Human Cell lysate - whole cell (Hek293T cells)
Gel Running Conditions
Reduced Denaturing (7.5% SDS gel)
Loading amount
30 µg
Treatment
Transfected with Tat containing plasmid
Specification
Hek293T cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1X TBST buffer
Dilution
1/800
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: anti-mouse IgG-HRP conjugated
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Thermo Scientific West Pico Chemilumine
Exposure
30 second(s)
Bands
Specific: 260 kDa
Additional data
Additional Notes
Predicted mol weight was 217 but detects a band at 260 with a few smaller bands. However, this blot was clean with no truncations
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投稿 Jan 20 2016