Anti-RNA Helicase A 抗体 [2274D5a] (ab53668)
Key features and details
- Mouse monoclonal [2274D5a] to RNA Helicase A
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG2a
製品の概要
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製品名
Anti-RNA Helicase A antibody [2274D5a]
RNA Helicase A 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2274D5a] to RNA Helicase A -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Recombinant fragment -
免疫原
RNA helicase A recombinant fragment (Human)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.0204% Monobasic dihydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate, 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
ab53668 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. -
ポリ/モノ
モノクローナル -
クローン名
2274D5a -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53668の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 141 kDa.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 141 kDa. |
ターゲット情報
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機能
Unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Component of the CRD-mediated complex that promotes MYC mRNA stability. -
配列類似性
Belongs to the DEAD box helicase family. DEAH subfamily.
Contains 2 DRBM (double-stranded RNA-binding) domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
ドメイン
The MTAD domain mediates interaction with the RNA polymerase II holoenzyme. The NTD domain is necessary and sufficient for nucleo-cytoplasmic shuttling and interaction with HRMT1L2 and SMN1. -
翻訳後修飾
Methylated. HRMT1L2 mediated methylation of undefined Arg residues in the NTD is required for nuclear localization.
May be phosphorylated by PRKDC/XRCC7. Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Can shuttle between nucleus and cytoplasm. - Information by UniProt
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別名
- ATP dependent RNA helicase A antibody
- ATP-dependent RNA helicase A antibody
- DDX 9 antibody
see all
画像
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Anti-RNA Helicase A antibody [2274D5a] (ab53668) + Recombinant immunising protein
Predicted band size: 141 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab53668 は論文での使用が確認できていません。