Application
Western blot
Sample
Human Cell lysate - nuclear (Raji, Hct116)
Specification
Raji, Hct116
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 5 % TBST-milk
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ecl
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: ca. 70 kDa
Positive control
raji as positive control
Additional data
Additional Notes
specific, clear. would recommend and use again!
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Dec 07 2007