Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
56000 cells
Treatment
Transfeceted for 24 hours
Specification
HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 21°C · Diluent: 1% Skim milk in PBS-T (0.02% Tween 20)
Dilution
1/2000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Goat anti Rabbit-HRP conjugate (Jackson IR)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Negative control
Untransfected HeLa cell whole cell lysate
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 60 kDa
Positive control
Whole cell lysate of HeLa cells transfected with a mammalian expression vector encoding mRFP fused to a human protein.
Additional data
Additional Notes
mStrawberry is a monomeric red fluorescent protein adapted from fluorescent proteins found in Discosoma sp. Originally described here - http://www.ncbi.nlm.nih.gov/pubmed/15558047
Lane 1 - Untransfected HeLa cell lysate
Lane 2 - mStrawberry transfected HeLa cell lysate
Lane 3 - mRFP transfected HeLa cell lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Sep 23 2015