製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RDH10
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human RDH10.

    (Peptide available as ab90185.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in human skeletal muscle tissue lysate and the following whole cell lysates: HepG2; HEK293.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab80891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 38 kDa).

ターゲット情報

画像

  • All lanes : Anti-RDH10 antibody (ab80891) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 34 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

プロトコール

参考文献

This product has been referenced in:
  • Sahu B  et al. Conditional Ablation of Retinol Dehydrogenase 10 in the Retinal Pigmented Epithelium Causes Delayed Dark Adaption in Mice. J Biol Chem 290:27239-47 (2015). Read more (PubMed: 26391396) »

See 1 Publication for this product

レビューと Q&A

Merci de nous avoir contactés.

A notre connaissance, ces deuxanticorps anti-RDH10n'ontété testés qu'en Western Blot et sur échantillons humains. Toutes nos garanties d'applications et d'espèces test&...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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