Anti-RCC2 抗体 (ab70788)
Key features and details
- Rabbit polyclonal to RCC2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-RCC2 antibody
RCC2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RCC2 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan, Bat, Elephant -
免疫原
Synthetic peptide corresponding to a region between residue 475 and the C-terminus (residue 522) of human RCC2. (NP_061185.1).
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ポジティブ・コントロール
- HeLa and 293T whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70788の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (2) |
1/2000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
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特記事項 |
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IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/2000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). |
ターゲット情報
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機能
Required for completion of mitosis and cytokinesis. May function as a guanine nucleotide exchange factor for the small GTPase RAC1. -
配列類似性
Contains 7 RCC1 repeats. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus > nucleolus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Appears in the nucleus at G2, then concentrates at the inner centromere region of chromosomes during prophase. Redistributes to the midzone of the mitotic spindle during anaphase. Here, the protein covers the entire equatorial diameter from cortex to cortex. - Information by UniProt
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参照データベース
- Entrez Gene: 55920 Human
- Entrez Gene: 108911 Mouse
- Omim: 609587 Human
- SwissProt: Q9P258 Human
- SwissProt: Q8BK67 Mouse
- Unigene: 380857 Human
- Unigene: 253 Mouse
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別名
- KIAA1470 antibody
- Protein RCC2 antibody
- RCC 2 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin basal cell carcinoma (left) and mouse CT26 colon carcinoma (right) tissues labelling RCC2 with ab70788 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB.
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All lanes : Anti-RCC2 antibody (ab70788) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 56 kDa
Observed band size: 56 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab70788 は 2 報の論文で使用されています。
- Gu G et al. A pan‑cancer analysis of RCC2 and its interaction with HMGA2 protein in an in vitro model of colorectal cancer cells. Exp Ther Med 24:725 (2022). PubMed: 36340602
- Atkinson SJ et al. The ß3-integrin endothelial adhesome regulates microtubule-dependent cell migration. EMBO Rep 19:N/A (2018). PubMed: 29794156