Anti-RC3H1 抗体 (ab70196)
Key features and details
- Rabbit polyclonal to RC3H1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RC3H1 antibody
RC3H1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RC3H1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat -
免疫原
Synthetic peptide corresponding to a region between C terminal residues 1075 and 1133 of human RC3H1 (NP_742068.1)
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ポジティブ・コントロール
- HeLa and 293T whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab70196 was affinity purified using an epitope specific to RC3H1 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70196の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | ||
IP |
特記事項 |
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WB: 1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 126 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Represses ICOS function, an essential costimulatory receptor for follicular T-cells. May act as an ubiquitin ligase that regulates ubiquitin-dependent control of AU-rich mRNAs by AUF1/HNRPD and HUR/ELAVL1. -
組織特異性
Widely expressed. Expressed at higher level in cerebellum, spleen, ovary and liver. -
配列類似性
Contains 1 C3H1-type zinc finger.
Contains 1 RING-type zinc finger. -
細胞内局在
Cytoplasm. Localizes to cytosolic RNA granules, which are sites for regulating mRNA translation and decay, suggesting that it may bind RNA. - Information by UniProt
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参照データベース
- Entrez Gene: 149041 Human
- Omim: 609424 Human
- SwissProt: Q5TC82 Human
- Unigene: 30258 Human
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別名
- KIAA2025 antibody
- Rc3h1 antibody
- RC3H1_HUMAN antibody
see all
画像
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All lanes : Anti-RC3H1 antibody (ab70196) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 126 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds -
Western Blot showing Lane 2: ab70196 at 0.04µg/ml staining RC3H1 in HeLa whole cell lysates immunoprecipitated using ab70196 at 3µg/mg lysate (1 mg/IP; 1/4 of IP loaded/lane). Lane 1: an irrelevant antibody was used for immunoprecipitation. Detection: Chemiluminescence with an exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab70196 は 1 報の論文で使用されています。
- Hou Y et al. YTHDC1-mediated augmentation of miR-30d in repressing pancreatic tumorigenesis via attenuation of RUNX1-induced transcriptional activation of Warburg effect. Cell Death Differ 28:3105-3124 (2021). PubMed: 34021267