Application
ChIP
Sample
Rat Cell lysate - nuclear (Cardiac fibroblasts)
Negative control
ChIP with rabbit IgG
Specification
Cardiac fibroblasts
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Positive control
I didn't use a positive control
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: provided with the kit
Dilution
1/20
Additional data
Additional Notes
I added 5mc-liter of antibody to 100mc-liter of dilution buffer.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mrs. Tal Golan Lagziel
Verified customer
投稿 Oct 31 2017