Application
Western blot
Sample
Human Cell lysate - whole cell (hESC)
Gel Running Conditions
Non-reduced Denaturing (4-20%)
Loading amount
20 µg
Specification
hESC
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
10 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-5%BSA
Dilution
1/2000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Goat anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Detection
Detection method
ECL Regular
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 130 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Miguel Fidalgo
Verified customer
投稿 Nov 13 2015