Anti-RASA1 抗体 [EP536Y] (ab40677)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP536Y] to RASA1
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-RASA1 antibody [EP536Y]
RASA1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP536Y] to RASA1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Rat, Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide within Human RASA1 aa 250-350 (internal sequence). The exact sequence is proprietary. From near the SH3 domain.
Database link: P20936 -
ポジティブ・コントロール
- WB: Rat brain tissue lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP536Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab40677の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000. Detects a band of approximately 140 kDa (predicted molecular weight: 96 kDa).
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IHC-P |
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/2000. Detects a band of approximately 140 kDa (predicted molecular weight: 96 kDa). |
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21. -
組織特異性
In placental villi, detected only in the trophoblast layer (cytotrophoblast and syncytiotrophoblast). Not detected in stromal, endothelial or Hofbauer cells (at protein level). -
関連疾患
Note=Mutations in the SH2 domain of RASA seem to be oncogenic and cause basal cell carcinomas.
Defects in RASA1 are the cause of capillary malformation-arteriovenous malformation (CMAVM) [MIM:608354]. CMAVM is a disorder characterized by atypical capillary malformations that are multiple, small, round to oval in shape and pinkish red in color. These capillary malformations are associated with either arteriovenous malformation, arteriovenous fistula, or Parkes Weber syndrome.
Defects in RASA1 are a cause of Parkes Weber syndrome (PKWS) [MIM:608355]. PKWS is a disorder characterized by a cutaneous flush with underlying multiple micro-arteriovenous fistulas, in association with soft tissue and skeletal hypertrophy of the affected limb. -
配列類似性
Contains 1 C2 domain.
Contains 1 PH domain.
Contains 1 Ras-GAP domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
翻訳後修飾
The N-terminus is blocked. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 5921 Human
- Entrez Gene: 218397 Mouse
- Entrez Gene: 25676 Rat
- Omim: 139150 Human
- SwissProt: P20936 Human
- SwissProt: P50904 Rat
- Unigene: 664080 Human
- Unigene: 12223 Rat
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別名
- CM AVM antibody
- CMAVM antibody
- DKFZp434N071 antibody
see all
画像
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Anti-RASA1 antibody [EP536Y] (ab40677) at 1/2000 dilution + Rat brain tissue lysate at 10 µg
Predicted band size: 96 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa (possible cleavage fragment) -
Immunohistochemical analysis of paraffin embedded human ovary carcinoma using ab40677 diluted 1:50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab40677 は 10 報の論文で使用されています。
- Jiang LN et al. Identification of the circ_PRKDC/miR-20a-3p/RASA1 axis in regulating HaCaT keratinocyte migration. Wound Repair Regen 30:282-291 (2022). PubMed: 34897876
- Sun X et al. Exosome-transmitted miRNA-335-5p promotes colorectal cancer invasion and metastasis by facilitating EMT via targeting RASA1. Mol Ther Nucleic Acids 24:164-174 (2021). PubMed: 33767913
- Cao Y et al. RNA-binding protein QKI suppresses breast cancer via RASA1/MAPK signaling pathway. Ann Transl Med 9:104 (2021). PubMed: 33569406
- Jing L et al. MicroRNA-4530 suppresses cell proliferation and induces apoptosis by targeting RASA1 in human umbilical vein endothelial cells. Mol Med Rep 19:3393-3402 (2019). PubMed: 30864691
- Kim DY et al. Colorectal cancer cells differentially impact migration and microRNA expression in endothelial cells. Oncol Lett 18:6361-6370 (2019). PubMed: 31814846
- Chen YL et al. Down-regulation of RASA1 Is Associated with Poor Prognosis in Human Hepatocellular Carcinoma. Anticancer Res 37:781-785 (2017). PubMed: 28179330
- Huang J et al. Co-expression and significance of Dok2 and Ras p21 protein activator 1 in breast cancer. Oncol Lett 14:5386-5392 (2017). PubMed: 29098030
- Teng HW et al. Protein tyrosine phosphatase 1B targets PITX1/p120RasGAP thus showing therapeutic potential in colorectal carcinoma. Sci Rep 6:35308 (2016). PubMed: 27752061
- Ying W et al. MicroRNA-223 is a crucial mediator of PPAR?-regulated alternative macrophage activation. J Clin Invest 125:4149-59 (2015). PubMed: 26436647
- Du C et al. Hypoxia-inducible MiR-182 promotes angiogenesis by targeting RASA1 in hepatocellular carcinoma. J Exp Clin Cancer Res 34:67 (2015). WB, IHC ; Human . PubMed: 26126858