Anti-RARRES1 抗体 (ab76530)
Key features and details
- Rabbit polyclonal to RARRES1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RARRES1 antibody
RARRES1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RARRES1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to RARRES1 aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab87448) -
ポジティブ・コントロール
- Recombinant Human RARRES1 protein (ab114592) can be used as a positive control in WB. This antibody gave a positive signal in the following lysates: Human Liver Tissue; Human Kidney Tissue; HepG2 Whole Cell; HEK293 Whole Cell
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76530の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 33 kDa).
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 33 kDa). |
ターゲット情報
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配列類似性
Belongs to the protease inhibitor I47 (latexin) family. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 5918 Human
- Omim: 605090 Human
- SwissProt: P49788 Human
- Unigene: 131269 Human
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別名
- 5430417P09Rik antibody
- AI662122 antibody
- RAR-responsive protein TIG1 antibody
see all
画像
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All lanes : Anti-RARRES1 antibody (ab76530) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Human kidney tissue lysate - total protein (ab30203)
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Additional bands at: 62 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
RARRES1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab76530 は 2 報の論文で使用されています。
- Pang B et al. Landscape of tumor suppressor long noncoding RNAs in breast cancer. J Exp Clin Cancer Res 38:79 (2019). PubMed: 30764831
- Chen HL et al. Secretome survey of human plexiform neurofibroma derived Schwann Cells reveals a secreted form of the RARRES1 protein. Int J Mol Sci 13:9380-99 (2012). PubMed: 22942771