Anti-RAPH1 抗体 (ab121619)
Key features and details
- Rabbit polyclonal to RAPH1
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RAPH1 antibody -
製品の詳細
Rabbit polyclonal to RAPH1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human RAPH1 aa 1100 to the C-terminus (C terminal).
Database link: Q70E73-10 -
ポジティブ・コントロール
- Human duodenum tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab121619の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. pH 6
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特記事項 |
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ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. pH 6 |
ターゲット情報
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機能
Mediator of localized membrane signals. Implicated in the regulation of lamellipodial dynamics. Negatively regulates cell adhesion. -
組織特異性
Isoform RMO1-RAPH1 is ubiquitously expressed with highest levels in brain, heart, ovary and developing embryo. Isoform RMO1 is widely expressed with highest levels in liver. Low expression in B-cells. -
配列類似性
Belongs to the MRL family.
Contains 1 PH domain.
Contains 1 Ras-associating domain. -
細胞内局在
Cell membrane. Cell projection > lamellipodium. Cell projection > filopodium. Cytoplasm > cytoskeleton. Recruited to the membrane, via the PH domain, by the phosphoinositide, PI(3,4)P2. Colocalizes with ENAH/VASP at the tips of lamellipodia and filopodia. Also colocalizes with the pathogens, Vaccinia and Enteropathogenic E.coli (EPEC) at the interface between the pathogen and their actin. - Information by UniProt
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参照データベース
- Entrez Gene: 65059 Human
- Omim: 609035 Human
- SwissProt: Q70E73 Human
- Unigene: 471162 Human
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別名
- 9430025M21Rik antibody
- Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 18 protein antibody
- Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 9 protein antibody
see all
画像
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Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus, plasma membrane and cytoplasm. Recommended concentration of ab121619 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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ab121619 at 1/75 staining RALPH1 in Paraffin Embedded Human duodenum tissue. Note strong cytoplasmic positivity in glandular cells.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab121619 は 1 報の論文で使用されています。
- Roşianu F et al. Loss of NDR1/2 kinases impairs endomembrane trafficking and autophagy leading to neurodegeneration. Life Sci Alliance 6:N/A (2023). PubMed: 36446521