Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEPG2)
Specification
HEPG2
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Diluent: 1% serum, 0.1% Triton, 0.1% BSA in PBS
Dilution
1/100
Secondary antibody
Dilution
1/250
Name
Non-Abcam antibody was used: alexa fluor 488 donkey anti rabbit
Host species: Human
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Human
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Abcam response
Thank you for providing the testing image. This antibody is guaranteed in ICC application. We would welcome furhter Abreviews regarding this antibody usage in immunohistochemistry.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Nov 18 2015