Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Thermo Scientific Starting Block T20 Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH6.0
Sample
Human Tissue sections (esophagus)
Specification
esophagus
Permeabilization
No
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1xPBS+0.1%BSA+0.2%TritonX-100
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: Biotinylated anti-rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
In normal esophagus, RANTES is expressed in the basal and suprabasal epithelial layers. Low level staining in the dermal layer is immune cell-derived. Magnified 10x (top) and 40x (bottom).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Tatiana Karakasheva
Verified customer
投稿 Oct 24 2014