Anti-RAGE 抗体 [1C1] (ab54741)
Key features and details
- Mouse monoclonal [1C1] to RAGE
- Suitable for: WB, IP, IHC-P
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2a
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-RAGE antibody [1C1]
RAGE 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [1C1] to RAGE -
由来種
Mouse -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Human, Recombinant fragment -
免疫原
Recombinant full length protein (GST-tag) corresponding to Human RAGE aa 23-405. Full length mature chain, without signal peptide.
Database link: Q15109 -
ポジティブ・コントロール
- WB: Human kidney tissue lysate. RAGE transfected HEK-293T cell lysate. Recombinant RAGE protein. Flow Cytometry: Jurkat cells. IHC-P: Human lung tissue.
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特記事項
This product was changed from ascites to tissue culture supernatant on 13th Feb 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: PBS
In 1x PBS -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
1C1 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab54741の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (3) |
Use at an assay dependent concentration. Predicted molecular weight: 43 kDa.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein in Western blot |
IP |
Use at an assay dependent concentration.
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IHC-P | (1) |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 43 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein in Western blot |
IP
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. -
組織特異性
Endothelial cells. -
配列類似性
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 177 Human
- Omim: 600214 Human
- SwissProt: Q15109 Human
- Unigene: 534342 Human
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別名
- Advanced glycosylation end product-specific receptor antibody
- Ager antibody
- DAMA 358M23.4 antibody
see all
画像
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Anti-RAGE antibody [1C1] (ab54741) at 1 µg/ml + Human kidney tissue lysate
Developed using the ECL technique.
Predicted band size: 43 kDaThis image was generated using the ascites version of the product.
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ab54741 (1 µg/ml) staining RAGE in human lung using an automated system (DAKO Autostainer Plus). Using this protocol there is membrane staining throughout the alveloli.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.This image was generated using the ascites version of the product.
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All lanes : Anti-RAGE antibody [1C1] (ab54741) at 1 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) RAGE transfected cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) non-transfected cell lysate
Developed using the ECL technique.
Predicted band size: 43 kDaThis image was generated using the ascites version of the product.
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Anti-RAGE antibody [1C1] (ab54741) at 1 µg/ml + tagged recombinant protein immunogen
Developed using the ECL technique.
Predicted band size: 43 kDaPredicted band size of immunogen is 68 kDa
This image was generated using the ascites version of the product.
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Immunoprecipitation of RAGE transfected lysate using ab54741 and Protein A Magnetic Bead. Immunoblotted with a rabbit polyclonal anti-RAGE antibody.
This image was generated using the ascites version of the product.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (19)
ab54741 は 19 報の論文で使用されています。
- Ahmed S et al. Nitrative DNA damage in lung epithelial cells exposed to indium nanoparticles and indium ions. Sci Rep 10:10741 (2020). PubMed: 32612147
- Tang ST et al. Glucagon-like peptide-1 attenuates endothelial barrier injury in diabetes via cAMP/PKA mediated down-regulation of MLC phosphorylation. Biomed Pharmacother 113:108667 (2019). PubMed: 30852419
- Nonaka K et al. 6-Shogaol Inhibits Advanced Glycation End-Products-Induced IL-6 and ICAM-1 Expression by Regulating Oxidative Responses in Human Gingival Fibroblasts. Molecules 24:N/A (2019). PubMed: 31619000
- Wu Y et al. S100B is required for maintaining an intermediate state with double-positive Sca-1+ progenitor and vascular smooth muscle cells during neointimal formation. Stem Cell Res Ther 10:294 (2019). PubMed: 31547879
- de Carvalho GC et al. RAGE and CCR7 mediate the transmigration of Zika-infected monocytes through the blood-brain barrier. Immunobiology 224:792-803 (2019). PubMed: 31493920