Application
Western blot
Sample
Mouse Tissue lysate - whole (Whole lung tissue lysate)
Gel Running Conditions
Reduced Denaturing (4-12% BT gel)
Loading amount
25 µg
Specification
Whole lung tissue lysate
Blocking step
Odyssey Blocking Buffer (PBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: IRDye® 800CW Goat anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
Infrared Scanner
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: around 50 kDa Non-specific: 30, 15 kDa
Additional data
Additional Notes
0.45 uM Nitrocellulose membrane, 1X RIPA buffer + protein and phosphatase inhibitor cocktails
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 25 2016