Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Non-reduced Denaturing (12.5% SDS-PAGE)
Sample
Mouse Tissue lysate - whole (mouse lung)
Specification
mouse lung
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% skim milk dilution in TBST
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/7500
Detection
Detection method
ECL+
Bands
Specific: 45-50 kDa Non-specific: 36, 30, 26 kDa
Exposure
30 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 Sep 12 2014