Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (RAW 264.7)
Specification
RAW 264.7
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: PBS-T
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: Alex Fluor 488 Donkey anti-Rabbit IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200
Additional data
Additional Notes
Cells were serum starved overnight and treated with BSA-AGE for 60 minutes.
Cytoplasmic/membranous staining was not observed unless non-specific nuclear staining was oversaturated. Imaging with no-primary control revealed no, non-specific secondary binding.
Abcam response
Customer has received this antibody with Abtrial offer for testing in IP. We are waiting for IP results in order to make further decision. In case the antibody fails in IP it will be replaced with a new one.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Oct 18 2013