Anti-RACK1 抗体 (ab62735)
Key features and details
- Rabbit polyclonal to RACK1
- Suitable for: ICC/IF, WB, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-RACK1 antibody
RACK1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RACK1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IPmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Chicken, Cow, Pig, Xenopus laevis, Cynomolgus monkey -
免疫原
Synthetic peptide:
TEQMTLRGT LKGHNG - C
, conjugated to KLH by a C terminal Cysteine residue linker, corresponding to N terminal amino acids 2-16 of Human RACK1 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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精製度
IgG fraction -
特記事項(精製)
The antiserum was fractionated and further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab62735の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use at an assay dependent concentration.
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WB | (2) |
1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 35 kDa).
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IP | (1) |
Use at an assay dependent concentration.
Use 5-10 µL. |
特記事項 |
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ICC/IF
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 35 kDa). |
IP
Use at an assay dependent concentration. Use 5-10 µL. |
ターゲット情報
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機能
Seems to bind protein kinase C acting as an intracellular receptor to anchor the activated PKC to the cytoskeleton. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and ITGB1, serves as a platform for SRC activation or inactivation. May play an important role in the developing brain and neuronal differentiation. -
配列類似性
Belongs to the WD repeat G protein beta family.
Contains 7 WD repeats. -
ドメイン
The WD repeats domain 5 mediates interaction with TRIM63. -
細胞内局在
Cell membrane. Located on plasma membrane of neuroblastoma NMB7 cells. - Information by UniProt
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参照データベース
- Entrez Gene: 417044 Chicken
- Entrez Gene: 327682 Cow
- Entrez Gene: 10399 Human
- Entrez Gene: 14694 Mouse
- Entrez Gene: 397605 Pig
- Entrez Gene: 83427 Rat
- Omim: 176981 Human
- SwissProt: P63247 Chicken
see all -
別名
- Cell proliferation-inducing gene 21 protein antibody
- GBLP_HUMAN antibody
- Gnb2-rs1 antibody
see all
画像
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All lanes : Anti-RACK1 antibody (ab62735) at 1/500 dilution
Lane 1 : Extract from hek-293T cell line
Lane 2 : Extract from HeLa cell line
Lane 3 : Extract from MCF7 cell line
Lane 4 : Extract from U2OS cell line
Lane 5 : Extract from Jurkat cell line
Lane 6 : Extract from RAT1 cell line
Lane 7 : Extract from PC12 cell line
Lane 8 : Extract from NIH3T3 cell line
Secondary
All lanes : Goat Anti-Rabbit IgG
Developed using the ECL technique.
Predicted band size: 35 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab62735 は 11 報の論文で使用されています。
- Wan L et al. Translation stress and collided ribosomes are co-activators of cGAS. Mol Cell 81:2808-2822.e10 (2021). PubMed: 34111399
- Li H et al. Repetitive Transcranial Magnetic Stimulation Alleviates Neurological Deficits After Cerebral Ischemia Through Interaction Between RACK1 and BDNF exon IV by the Phosphorylation-Dependent Factor MeCP2. Neurotherapeutics 17:651-663 (2020). PubMed: 31912469
- Chen Y et al. O-GlcNAcylated c-Jun antagonizes ferroptosis via inhibiting GSH synthesis in liver cancer. Cell Signal 63:109384 (2019). PubMed: 31394193
- Zhu L et al. Upregulated RACK1 attenuates gastric cancer cell growth and epithelial-mesenchymal transition via suppressing Wnt/ß-catenin signaling. Onco Targets Ther 12:4795-4805 (2019). PubMed: 31417279
- Lee JS et al. RACK1 mediates rewiring of intracellular networks induced by hepatitis C virus infection. PLoS Pathog 15:e1008021 (2019). PubMed: 31525236