Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HEK 293 cells transfected with hRab27a-GFP)
Total protein in input
20 µg
Specification
HEK 293 cells transfected with hRab27a-GFP
Immuno-precipitation step
Protein G
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1%TX-100, 10% glycerol, 1XPBS
Additional data
Additional Notes
1. lysate of HEK 293 cells expressing Rab27a-GFP
2. IP with anti Rab27a Ab
3. Not bound fraction
2. IP with anti Rab27a Ab
3. Not bound fraction
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
HERR DR. Vladimir Milenkovic
Verified customer
投稿 Feb 29 2008